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fe 221 amplifier  (ADInstruments)


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    ADInstruments fe 221 amplifier
    Fe 221 Amplifier, supplied by ADInstruments, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fe 221 amplifier/product/ADInstruments
    Average 95 stars, based on 95 article reviews
    fe 221 amplifier - by Bioz Stars, 2026-04
    95/100 stars

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    <t>MiR-221/222</t> sponge ALL cells have increased p27, with reduced proliferation and chemosensitivity. A, Schematic of lentiviral miRNA sponge construct along with western blot of p27 abundance alterations within Nalm-27 and REH miR-221/222 sponge cells. Values depict fold change relative to empty vector control and normalized to housekeeping gene GAPDH. B, Immunofluorescent images of p27 in miR-221/222 sponge cells compared to empty vector control. C, Proliferation index was measured 4 days after cell retention dye addition in cells described in A. D, The viability of these cells after 48 hour exposure to Ara-C and VCR were measured by trypan blue exclusion. Error bars represent standard error of the mean with samples performed in triplicate. *, P < 0.05. Western blot, immunofluorescent images and bar graphs are representative data from 3 independent experiments. Scale bars, 10 μm. EV, empty vector; Sp, sponge; Ara-C, cytarabine; VCR, vincristine.
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    Image Search Results


    MiR-221/222 sponge ALL cells have increased p27, with reduced proliferation and chemosensitivity. A, Schematic of lentiviral miRNA sponge construct along with western blot of p27 abundance alterations within Nalm-27 and REH miR-221/222 sponge cells. Values depict fold change relative to empty vector control and normalized to housekeeping gene GAPDH. B, Immunofluorescent images of p27 in miR-221/222 sponge cells compared to empty vector control. C, Proliferation index was measured 4 days after cell retention dye addition in cells described in A. D, The viability of these cells after 48 hour exposure to Ara-C and VCR were measured by trypan blue exclusion. Error bars represent standard error of the mean with samples performed in triplicate. *, P < 0.05. Western blot, immunofluorescent images and bar graphs are representative data from 3 independent experiments. Scale bars, 10 μm. EV, empty vector; Sp, sponge; Ara-C, cytarabine; VCR, vincristine.

    Journal: Molecular cancer research : MCR

    Article Title: Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

    doi: 10.1158/1541-7786.MCR-15-0474

    Figure Lengend Snippet: MiR-221/222 sponge ALL cells have increased p27, with reduced proliferation and chemosensitivity. A, Schematic of lentiviral miRNA sponge construct along with western blot of p27 abundance alterations within Nalm-27 and REH miR-221/222 sponge cells. Values depict fold change relative to empty vector control and normalized to housekeeping gene GAPDH. B, Immunofluorescent images of p27 in miR-221/222 sponge cells compared to empty vector control. C, Proliferation index was measured 4 days after cell retention dye addition in cells described in A. D, The viability of these cells after 48 hour exposure to Ara-C and VCR were measured by trypan blue exclusion. Error bars represent standard error of the mean with samples performed in triplicate. *, P < 0.05. Western blot, immunofluorescent images and bar graphs are representative data from 3 independent experiments. Scale bars, 10 μm. EV, empty vector; Sp, sponge; Ara-C, cytarabine; VCR, vincristine.

    Article Snippet: Primer sets designed to amplify mature miR-221/222 were purchased from Life Technologies.

    Techniques: Construct, Western Blot, Plasmid Preparation

    Primary transcript levels of miR-221/222 are unaltered in BMM influenced ALL. Fold change of the pri-miRNA of miR-221/222 and mature miR-221 was measured by qRT-PCR in Nalm-27 and REH cells co-cultured with BMSC or HOB relative to cells cultured with media only. Error bars represent standard deviation of the mean with samples performed in triplicate. *, P < 0.05. Results are representative data from 3 independent experiments. pri-miR, primary miRNA transcript; mature miR, mature miRNA.

    Journal: Molecular cancer research : MCR

    Article Title: Bone Marrow Microenvironment Niche Regulates miR-221/222 in Acute Lymphoblastic Leukemia

    doi: 10.1158/1541-7786.MCR-15-0474

    Figure Lengend Snippet: Primary transcript levels of miR-221/222 are unaltered in BMM influenced ALL. Fold change of the pri-miRNA of miR-221/222 and mature miR-221 was measured by qRT-PCR in Nalm-27 and REH cells co-cultured with BMSC or HOB relative to cells cultured with media only. Error bars represent standard deviation of the mean with samples performed in triplicate. *, P < 0.05. Results are representative data from 3 independent experiments. pri-miR, primary miRNA transcript; mature miR, mature miRNA.

    Article Snippet: Primer sets designed to amplify mature miR-221/222 were purchased from Life Technologies.

    Techniques: Quantitative RT-PCR, Cell Culture, Standard Deviation